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Test Code Lab5728 NT-proBNP 

Performing Laboratory

St. Louis Children's Hospital Laboratory - Chemistry

Specimen Requirements

Patient Preparation: Samples should not be taken from patients receiving therapy with high biotin doses (i.e., >5 mg/day) until at least 8 hours following the last biotin administration

Collection Container/Tube:

Preferred: Mint green-top Lithium Heparin (MNTGRN LiHep) tube

Acceptable: Green samplette/microtainer, plain red-top tube, Gold SST gel tube, or Lavender-top (EDTA) tube

Submission Container/Tube: If specimen is coming from outside facility and collected in a microtainer, plain red-top tube, or lavender-top tube, aliquot processed sample into a plastic vial for transport (labeling with specimen type).

Specimen Collection Volume: 1.0 mL (minimum 0.6 mL)

Special Instructions: None

 

Sample Rejection

Specimens that are grossly hemolyzed

Mislabled or unlabeled specimens

Logistics

Test Availability: Monday-Sunday

Cut-off time: None, performed as received

Turnaround Time: Same Day; 2 hours from receipt in performing lab

Reference Values

Reference Interval: 

≤ 10 days: ≤ 10000 pg/mL                                                 
11 days-31 days: ≤ 5000 pg/mL
31 days - 1 year: ≤ 1000 pg/mL
1-2 years: ≤ 700 pg/mL
2-75 years: ≤ 300 pg/mL
≥ 75 years: ≤ 450 pg/mL

Critical Value: None established

 

Methodology

Electrochemiluminescence Immunoassay (ECLIA)

Limitations

  • In rare cases, interference due to extremely high titers of antibodies to analyte-specific antibodies (such as HAMA), streptavidin or ruthenium can occur.

Additional Information

FOR SLCH CORE LABORATORY USE ONLY

Lab Processing Instructions: Centrifuge to separate. If specimen is collected in microtainer, plain red-top tube, or lavender-top tube aliquot sample into a Roche false-bottom tube for testing (labeling aliquot with specimen type).

Processed Volume: 0.5 mL (minimum 0.3 mL) plasma/serum

Analyte Stability:

Specimen Type Temperature Time
Whole Blood (unprocessed)  Ambient NA
Plasma/Serum (processed) Refrigerated (preferred)   6 days
  Ambient  3 days
  Frozen  24 months    

Test Classification and CPT Code

83880

LOINC

83107-3

Last Reviewed

06/2025