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HelpTest Code LAB7795 TB (M. tuberculosis) Test, T-SPOT, Blood
Additional Codes
| Quest Diag Test ID |
|---|
| 37737 |
Performing Laboratory
St. Louis Children's Hospital Sendouts to Quest Diagnostics (Quest RLI)
Specimen Requirements
Patient Preparation: None
Container/Tube
Preferred/Acceptable: Greiner 9 mL lithium heparin (green-top) tube provided by SLCH Core lab. Call laboratory at 314-454-4268 for specific collection tube needed.
Collection Volume:
Adults and children ≥10 years: 6 mL whole blood
Pediatric 2-9 years of age: 4 mL whole blood
Pediatric <2 years of age: 2 mL whole blood
Immunocompromised Adults and children ≥10 years: 9 mL whole blood
Submission Container/Tube: If specimen is coming from outside facility, send whole blood in original collection container.
Special Instructions: Immediately invert tube gently 8-10 times after collection to ensure proper mixing of blood and anticoagulant (this will avoid specimen clotting). Do not overfill.
Sample Rejection
Mislabeled or unlabeled specimens
Clotted specimens
Specimens collected between the hours of 1700-0300 Monday-Friday or any specimen collected on a Saturday, Sunday, or day of/before a holiday
Specimens collected in EDTA tubes
Hemolyzed specimens
Logistics
Test Availability: Monday-Friday between 0300-1700 ONLY!
Cut-off time: 1700
Test set-up: Monday-Friday
Turnaround Time: 2-4 days
Reference Values
Reference Interval: Negative
Critical Value: None established
Methodology
Enzyme linked Immunosorbent (ELISPOT) Assay
Limitations
- NA
Additional Information
FOR SLCH CORE LABORATORY USE ONLY
Lab Processing Instructions: DO NOT SPIN Send in original collection tube, keeping at ambient temperature until packaged for transport.
Processed Volume:
2 years: minimum 2.0 mL
2-10 years: minimum 4.0 mL
>or=10 years: minimum 6.0 mL
Transport Temperature: Ambient
Analyte Stability:
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood (unprocessed) | Ambient | 54 hours |
| Refrigerated | unacceptable | |
| Frozen | unacceptable |
Specimen must be received at performing lab within 39 hours of collection
NOTES:
- Specimens should be sent out Monday-Friday
- Packaged specimens will be picked up by Oxford courier at 1815 Monday-Friday
- 9.0 mL Greiner tubes for collection are stored in sendouts
Test Classification and CPT Code
86481
Test Classification: This test has been cleared or approved for diagnostic use by the U.S. Food and Drug Administration.
LOINC
- 71773-6
Last Reviewed
08/2025