Home
HelpTest Code LAB6532 Immunofixation Electrophoresis, Urine with Interpretation
Performing Laboratory
Barnes-Jewish Hospital Laboratory
Specimen Requirements
Submit only 1 of the following specimens:
Preferred
Specimen Type: Urine, 24 hour
Container/Tube: Plain brown urine container
Specimen Volume: Entire collection
Collection Instructions: No additive. Refrigerate specimen during collection.
Additional Information:
1. Starting and ending times of collection are required.
2. See Urine Collection in Special Instructions for additional information.
3. Collection periods <20 hours will be ordered as a timed-urine collection.
4. Collection periods >48 hours require approval from the Laboratory Medicine resident before analysis.
Specimen Type: Urine, random
Container/Tube: Urine Complete Cup Kit
Specimen Volume: 20 mL from a first-morning collection.
Collection Instructions: No additive.
Additional Information: See Urine Collection in Special Instructions for additional information.
Alternate
Specimen Type: Urine, timed collection
Container/Tube: Plain brown urine container
Specimen Volume: Entire collection
Collection Instructions: No additive. Refrigerate specimen during collection.
Additional Information:
1. Starting and ending times of collection are required.
2. See Urine Collection in Special Instructions for additional information.
3. Collection periods <20 hours will be ordered as a timed-urine collection.
4. Collection periods >48 hours require approval from the Laboratory Medicine resident before analysis.
Reference Values
No monoclonal protein detected.
Literature Reference:
Alper CA, Johnson AM. Immunofixation electrophoresis: a technique for the study of protein polymorphism. Vox Sang 1969;17:445-52.
Methodology
Gel Electrophoresis/Immunoprecipitation
Note: This test is automatically performed when #5309 Protein Electrophoresis, 24 Hour, Urine is ordered. A pathologist interpretation will be preformed, at an additional charge.
Note: Methods are not standardized. It is best to maintain a consistent method and laboratory. Caution should be used when quantitatively comparing results from different labs.
Literature Reference:
Whicher JT, Hawkins L, Higginson J. Clinical applications of immunofixation: a more sensitive technique for the detection of Bence-Jones protein. J Clin Pathol 988;33:779-80.
Additional Information
For BJH Laboratory Use Only
Alternate Tube:
Plain, brown urine container preferred; any clean container will be accepted.
Analyte Stability:
24 hours at ambient temperature is acceptable; refrigeration during collection is preferred.
Laboratory Processing Instructions:
Test performed in BJH Core Lab. If ordered with a urine protein electrophoresis; this is a duplicate and should be cancelled. If ordered alone; do not send an aliquot to Chemistry for total protein, and concentrate 100x. Keep a 10-mL urine specimen in refrigerator for possible future use. Place concentrate in appropriate rack.
Test Classification and CPT Coding
86335
Specimen Transport Temperature
Ambient
Day(s) Test Set Up
Monday through Friday
Cut-off time: 0800
Turnaround Time:
STAT: not available
Routine: 2 working days
LOINC Code Information
| Name | LOINC Code |
| Immunofixation, Urine Interpretation | 14896-5 |