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HelpTest Code LAB5029 Amikacin Level, Trough
Performing Laboratory
St. Louis Children's Hospital - Chemistry
Specimen Requirements
Patient Preparation: None
Container/Tube
Preferred: Mint green-top Lithium Heparin (MNTGRN LiHep) tube
Acceptable: Lavender-top (EDTA) tube or plain red-top tube
Collection Volume: 2.0 mL (minimum 1.0 mL)
Submission Container/Tube: If specimen is coming from outside facility and collected in a microtainer, Lavender-top (EDTA) tube, or plain red-top tube aliquot processed sample into a plastic vial for transport (labeling with specimen type).
Special Instructions: Collect immediately before next scheduled dose or no earlier than 1 hour prior to next dose (Trough levels for first dose should be collected just prior to next dose). Send to lab promptly.
Sample Rejection
Mislabeled or unlabeled specimens
Logistics
Test Availability: Monday-Sunday
Cut-off time: None; performed as received
Turnaround Time: Same day; 2 hours from receipt in performing lab
Reference Values
Therapeutic Range: <8.0 mcg/mL
Critical Values: >8.0 mcg/mL
Critical test results will be automatically called back initially and every 24 hours thereafter.
Methodology
Immunoassay (Kinetic interaction of microparticles in solution (KIMS) as measured by changes in light transmission).
Limitations
NA
Additional Information
FOR SLCH CORE LABORATORY USE ONLY
Lab Processing instructions: Centrifuge specimen to separate. If specimen is collected in a plain, red-top or Lavender-top (EDTA) tube, aliquot sample into a Roche false-bottom tube for testing (labeling with specimen type).
Processed Volume: 0.3 mL plasma/serum
Analyte Stability:
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood (unprocessed) | Ambient | 8 hours |
| Plasma/Serum (processed) | Refrigerated (preferred) | 2 days |
| Ambient | 8 hours | |
| Frozen | 4 weeks |
Test Classification and CPT Coding
80150
LOINC
3321-7
Last Reviewed
06/2025