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Test Code LAB494 Rapid Plasma Reagin (RPR), Blood

Testing Algorithm

RPR Quantitative and treponemal antibody confirmation testing will automatically be preformed at an additional charge whenever RPR Qualitative is reactive.

Performing Laboratory

St. Louis Children's Hospital Laboratory - Immunology/Serology

Specimen Requirements

Patient Preparation: None

Collection Container/Tube:

Preferred: Gold SST gel tube

Acceptable: Plain red-top tube or Lavender (EDTA) tube/or microtainer

Submission Container/Tube: If specimen is coming from outside facility and collected in a plain red-top tube or EDTA tube aliquot processed sample into a plastic vial for transport (labeling with specimen type).

Specimen Collection Volume: 2.0 mL (minimum 0.5 mL)

Special Instructions: None

 

Sample Rejection

Mislabled or unlabeled specimens

Specimen that are grossly hemolyzed and/or grossly turbid

Logistics

Test Availability: Monday-Sunday

Cut-off time: 1300

Turnaround Time: Same Day; next day if received after cut-off

Reference Values

Reference Interval: Non-reactive

Critical Value: None established

   NOTE: Physician or nurse will be called with notification of non-critical results that are "Reactive"

 

Methodology

Flocculation/Particle Agglutination card test

Limitations

  • NA

Additional Information

FOR SLCH CORE LABORATORY USE ONLY

Lab Processing Instructions: Centrifuge to separate. If specimen is collected in a plain red-top tube or EDTA tube aliquot sample into screw-cap,plastic vial for testing (labeling aliquot with specimen type).

Processed Volume: minimum 0.1 mL serum (or plasma)

Analyte Stability:

Specimen Type Temperature Time
Whole Blood (unprocessed)   Ambient NA
Plasma/Serum (processed) Refrigerated (preferred)     7 days    
  Ambient NA
  Frozen  NA      

NOTE:

  • EDTA plasma is only stable 24 hours
  • Do not test specimens that are grossly hemolyzed, contaminated or extremely turbid
  • CSF can not be tested by this method

Test Classification and CPT Code

86592

LOINC

20507-0

Last Reviewed

07/2025