Sign in →

Test Code LAB400 Bufulfan Pharmockinetics

Important Note

Advance notice is required for all sample shipments:

  • Tuesday to Saturday arrival: At least 48 hours in advance. Please call or email us to provide package tracking information.
  • Lab closes on Sundays and Mondays.
  • Holidays: Lab closes on all observed holidays and their very next days. Please call 206.606.7389 for details.

Performing Laboratory

St. Louis Children's Hospital Sendout to Seattle Cancer Care Alliance - PK Laboratory

Specimen Requirements

Specimen Type: Blood

Patient Preparation: None

Container/Tube        

Preferred/Acceptable:  Dark green-top (DRKGRN) Sodium Heparin Vacutainer®
Collection Volume: Draw minimum 1 mL (Pediatric) to 4 mL (Adult) of blood into 4-mL Sodium Heparin green top blood tubes.

Special Instructions: 

  • Busulfan degrades quickly at room temperature. Each sample should be kept in wet ice slurry or refrigerated at all time.
  • The REQUISITION SHEET for particular regimen, IV Q6 hr or test dose, IV Q24 hr, and Oral Q6 hr is provided in link below. Select which req is required for patient's regimen and print/complete. It is required to be sent with the samples. Patient identifiers must be matched with those on the collected samples mentioned above.
  • Requisition serves as physician’s order and must be signed by the attending physician or designee/caregiver according to the College American Pathologists (CAP) requirement. 
  • "Seattle Care Center Alliance" website for more information and requisition

Sample Rejection

Mislabeled or unlabeled specimens.

Logistics

Test Availability:  Must call lab to schedule in advance

Days set up by performing lab: same day received

Turnaround Time: 

  • The PK lab will usually release pharmacokinetics results on the same day as receipt of the samples. Average sample run-time is approximately 4 to 6 hours from receiving the package. 
  • Sample processing is prioritized by time zone differences or next dose due time to maximize the advantage of dose adjustments.

Reference Values

Reference Interval: None established

  • All results must be verbally delivered to attending physician, pharmacist, or caregiver who is a certified MD or PharmD. No exceptions.
  • After the verbal report, a hardcopy can be faxed to designated number(s).

Critical Value: None established

Methodology

Mass Spectometry

Limitations

  • NA

Additional Information

FOR SLCH CORE LABORATORY USE ONLY

Lab Processing Instructions: 

Each sample should be kept in wet ice slurry or refrigerated at all time. Centrifuge sample as soon as possible at 4 °C. Separate the plasma from each sample and transfer it to individual 5 mL plastic screw-cap vial. Immediately freeze plasma sample at -20 °C.

All samples have to be labeled with patient name, Medical Record #, date and actual clock time of the blood draw. Two unique identifiers and clock times are REQUIRED or samples can be rejected. All 6 samples and requisition should go to sendouts for shipment via Fed Ex.
Advanced notice of shipment is required for all samples shipped. Their lab is closed on Sunday and Monday. Also observed holidays and their very next day. Call 206-606-7389 if questions.

Samples must be shipped on a minimum of 3 kg of dry ice overnight Fed Ex. Samples that arrived thawed will not be analyzed.

Note: Please send samples to:

       Pharmacokinetics Laboratory
       Fred Hutchinson Cancer Center
       188 E Blaine Street, Suite 250
       Seattle, WA 98102

 

Lab Hours: Tuesday to Saturday, 8 a.m. to 5 p.m.
Lab closed on Sundays, Mondays and holidays

Phone: 206.606.7389
Fax: 206.606.7397
Email: pklab@fredhutch.org

Test Classification and CPT Code

80299

LOINC

NA

Last Reviewed

10/2025