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Test Code LAB1748 CBC with Auto Differential

Additional Codes

Includes: WBC, RBC, Hemoglobin, Hematocrit, Platelet count, RBC indices, and WBC differential

Testing Algorithm

If platelet count is <50 K/cumm then an IPF (immature platelet fraction) will be performed and reported at an additional charge

Performing Laboratory

St. Louis Children's Hospital - Hematology

Specimen Requirements

Patient Preparation: None

Container/Tube  
Preferred:
 Lavender-top (EDTA) tube
Acceptable: Lavender-top (EDTA) samplette/microtainer
Collection Volume: 

Vacutainer: 2.0 mL (minimum 0.5 mL)
Microtainer: Full tube (minimum 0.3 mL)                                               

Submission Container/Tube: If specimen is coming from outside facility, send whole blood in original collection container.
Special Instructions: Immediately invert tube gently 8-10 times after collection to ensure proper mixing of blood and anticoagulant (this will avoid specimen clotting). Do not overfill tube.

Sample Rejection

Mislabeled or unlabeled specimens

Clotted specimens

Specimens collected above an IV line (contaminated)

Logistics

Test Availability: Monday-Sunday
Cut-off time: None; performed as received
Turnaround Time: Same day; 2 hours from receipt in performing lab

Reference Values

Reference Interval: 

AGE WBC RBC HGB HCT NRBC Abs
  K/cumm M/cumm g/dL % K/cumm
<3 days: 9.0-30.0 3.9-6.0 14.5-22.5 45.0-66.0 0.00-2.50
3 days-2 weeks: 5.0-21.0 3.6-6.2 12.5-20.5 39.0-63.0 0.00-0.05
2 weeks-1 month: 5.0-20.0 3.0-5.4 10.0-18.0 31.0-55.0 0.00-0.01
1-6 months: 6.0-17.5 2.7-4.9 9.0-14.0 28.0-42.0 0.00-0.01
6  months-2 years: 6.0-17.5 3.7-5.3 10.5-13.5 33.0-39.0 0.00-0.01
2-6 years: 5.0-15.0 3.9-5.3 11.5-13.5 34.0-40.0 0.00-0.01
6-12 years: 4.5-13.5 4.0-5.2 11.5-15.5 35.0-45.0 0.00-0.01
>or=12 years, Female: 3.8-9.9 3.9-5.2 11.9-15.5 35.6-45.5 0.00-0.01
>or=12 years, Male: 3.8-9.9 4.3-5.8 13.0-17.5 38.9-50.3 0.00-0.01
AGE MCH MCHC MCV
  pg g/dL fL
<1 week: 28.0-40.0 28.0-38.0 88.0-123.0
1 week-1 month: 25.0-35.0 29.0-37.0 85.0-123.0
1-6 months: 25.0-35.0 29.0-37.0 74.0-115.0
6  months-2 years: 23.0-31.0 30.0-36.0 70.0-78.0
2-6 years: 24.0-30.0 32.7-35.5 75.0-87.0
6-12 years: 25.0-33.0 32.7-35.5 77.0-95.0
>or=12 years (all) 27.1-33.3 32.3-35.7 81.3-96.4
RDW CV  
<3 day: 15.0-20.0%
3 days-1 month: 13.0-19.0%
1-6 months: 12.0-16.0%
>or=6 months: 11.1-14.9%

Platelet count (all ages): 150,000-400,000 K/cumm

MPV (all ages): 9.1-12.3 fL

IPF (if applicable)
   <6 months: 1.5-7.0%
6 months-12 years: 1.1-5.0%
12-18 years: 1.6-6.5%
≥ 18 years: 1.6-10.1%               

DIFFERENTIAL

  <4 years 4-16 years >or=16 years
  K/cumm K/cumm K/cumm
Neutrophil Abs: 1.0-10.2 1.5-9.4 1.5-6.5
Lymphocyte Abs 1.2-11.5 1.0-7.2 0.8-3.3
Monocyte Abs 0.0-1.2 0.1-1.7 0.2-0.8
Eosinophil Abs 0.0-0.5 0.1-1.6 0.0-0.5
Basophil Abs 0.0-0.2 0.0-0.3 0.0-0.1
Immature Gran Abs 0.0-0.3 0.0-0.2 0.0-0.1

Critical: 

WBC  
<3 days: <2.0 K/cumm OR  >50.0 K/cumm  
>or=3 days: <1.0 K/cumm  OR  >50.0 K/cumm  
Hgb  
<12 years: <7.0 g/dL  
12-18 years: <6.0 g.dL  
>or=18 years: <6.5 g/dL  OR  >19.9 g/dL  
PLT - Inpatient:  
   <18 years: <25,000 K/cumm  OR  >1,000,000 K/cumm  
   ≥ 18 years: <20,000 K/cumm  OR  >1,000,000,K/cumm                          
PLT - Outpatient or ED patient:  
all ages: <50,000 K/cumm  OR  >1,000,000 K/cumm  
Differential  
NEUTAB or ANC: <0.5  
Blast: Any seen  
Promyelocyte: >20 cells seen  

All critical test results will be automatically called back initially and every 72 hours thereafter with the exception of outpatient or ED patient results, those will be called every time.

Methodology

see individual component

Limitations

  • Red cell fragments, microcytic RBCs or white cell cytoplasmic fragments may interfere with automated platelet counts.
  • Instrument may trigger a reflex to a manual smear. All specimens that reflex will have a slide smear be manually reviewed and if match auto-diff, the auto differential will be reported, otherwise a manual differential will be reported and resulted by lab.

Additional Information

FOR SLCH CORE LABORATORY USE ONLY

Lab Processing Instructions: DO NOT SPIN. Give specimen directly to Hematology bench
Processed Volume: NA
Analyte Stability:

Specimen Type Temperature Time
Whole Blood (unprocessed)  Ambient 24 hours
  Refrigerated     24 hours
  Frozen  unacceptable       

NOTE: refrigerated specimens must be warmed to room temperature before analysis. 

Test Classification and CPT Code

85027

85055 - IPF (if applicable)

LOINC

WBC: 6690-2

RBC: 789-8

Hgb: 718-7

Hct: 4544-3

Plt: 777-3

IPF (if applicable): 71693-6

Last Reviewed

07/2025