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Test Code LAB173 Testosterone, Free and Total, Serum

Additional Codes

Mayo Test ID
FFTFS

Performing Laboratory

Esoterix Endocrinology thru Mayo Medical Laboratory

Specimen Requirements

Includes: Total Testosterone, Free Testosterone, & SHBG

Patient Preparation: None

Collection Container/Tube:

Preferred: Gold SST gel tube

Acceptable: Plain red-top tube

Submission Container/Tube: If specimen is coming from outside facility aliquot processed sample into a plastic vial for transport within 1 hour.

Specimen Collection Volume: 4.0 mL (minimum 2.0 mL)

Special Instructions: Send to lab promptly

NOTE: Minimum volume does not allow for repeat analysis if necessary

Sample Rejection

Mislabled or unlabeled specimens

Logistics

Test Availability: Monday-Sunday

Cut-off time: None

Days set-up: Monday-Saturday

Turnaround Time: 8-16 days

Reference Values

Reference Interval: 

TESTOSTERONE TOTAL

PREMATURE INFANTS     MALE RANGE(ng/dL)      FEMALE RANGE(ng/dL)

26 – 28 Weeks, Day 4:            59 – 125                              5 – 16

31 – 35 Weeks, Day 4:            37 – 198                              5 – 22

FULL–TERM INFANTS

Newborns: 

Male: 75-400 ng/dL
    1 – 7 Months: Levels decrease rapidly the first week to 20 – 50 ng/dL, then increase to 60 – 400 ng/dL (Mean = 190) between 20 – 60 days. Levels then decline to prepubertal range of < 2.5 – 10 by seven months.

Female: 20-64 ng/dL
    1 – 7 Months: Levels decrease during the first month to <10 ng/dL and remain there until puberty.

PREPUBERTAL CHILDREN

Males (1 – 10 Years): < 2.5 – 10 ng/dL

Females (1 – 9 Years): < 2.5 – 10 ng/dL

PUBERTY
MALE       FEMALE      

TANNER STAGE

AGE (years)

RANGE (ng/dL)

 

TANNER STAGE

AGE (years)

RANGE (ng/dL)

 
1 <9.8 <2.5-10   1 <9.2 <2.5-10  
2 9.8-14.5 18-150   2 9.2-13.7 7-28  
3 10.7-15.4 100-320   3 10.0-14.4 15-35  
4 11.8-16.2 200-620   4 10.7-15.6 13-32  
5 12.8-17.3 350-970   5 118-18.6 20-38  

ADULTS ≥18 Years       RANGE (ng/dL)

Male:                                  264 – 916

Female:

  Premenopausal:                10 – 55

  Postmenopausal:               7 – 40

FREE TESTOSTERONE

MALES:

AGE (years)

RANGE (pg/mL)

  

FEMALES:

AGE (years)

RANGE (pg/mL)

 

1 - 15 day

1.5-31

    

1 - 15 day

0.5-2.5

 

31d - 2 mo

3.3-18

  

 

31d - 2 mo

0.1-1.3

 

3 - 4 mo

0.7-14

  

 

3 - 4 mo

0.3-1.1

 

5 - 6 mo

0.4-4.8

  

 

5 - 6 mo

0.2-0.6

Prepubertal Males and Females: 0.15-0.6 pg/mL

Adult Males:                           52-280 pg/mL

Adult Females:                        1.1-6.3 pg/mL

 

PERCENT FREE TESTOSTERONE (by DIALYSIS)

MALES:

AGE (years)

RANGE (pg/mL)

  

FEMALES:

AGE (years)

RANGE (pg/mL)

 

1 - 15 day

0.9-1.7

    

1 - 15 day

0.8-1.5

 

31d - 2 mo

0.4-0.8

  

 

31d - 2 mo

0.4-1.1

 

3 - 4 mo

0.4-1.1

  

 

3 - 4 mo

0.5-1.0

 

5 - 6 mo

0.4-1.0

  

 

5 - 6 mo

0.5-0.8

Prepubertal Males and Females: 0.4-0.9 pg/mL

Adult Males:                           1.5-3.2 pg/mL

Adult Females:                        0.8-1.4 pg/mL

 

Comprehensive values for free testosterone by dialysis throughout puberty are currently unavailable.

 

SEX HORMONE BINDING GLOBULIN (SHGB), SERUM*

AGE:                            RANGE (UOM: nmol/L)   

1 mo -23 months:                  60.0-252.0

Prepubertal (1-8 yr):              72.0-220.0 

Pubertal

    Males:                                16.0-100.0

    Females:                            36.0-125.0

Adult Males:     

20-49 years:                           16.5-55.9

>49 years:                              19.3-76.4

Adult Females: 

20-49 years:                            24.6-122.0

>49 years:                               17.3-125.0

*ASR - Analyte Specific Reagent

This test was developed and its performance characteristics determined by Esoterix. It has not been cleared or approved by the U.S. Food and Drug Administration. The FDA has determined that such clearance or approval is not necessary.

 

Critical Value: none established

 

Methodology

High-pressureLiquid Chromatography (HPLC)  tandem mass spectrometry;

equilibrium dialysis; electrochemiluminescence immunoassay (ECLIA)

Limitations

  • NA

Additional Information

FOR SLCH CORE LABORATORY USE ONLY

Lab Processing Instructions: Centrifuge specimen to separate within one hour of collection. Transfer specimen to a 5-mL screw-capped plastic transport vial.

Processed Volume: 2.0 mL (minimum 1.0 mL) serum

Transport temperature: Frozen

Analyte Stability:

Specimen Type Temperature Time
Whole Blood (unprocessed)   Ambient 1 hour
Plasma/Serum (processed) Refrigerated  48 hours
  Ambient  48 hours   
  Frozen (preferred)     NA    

NOTES: 

1.

Specimen must be separated within 1 hour of collection and removed from gel tube into a plastic vial for transport.

2.

Minimum volume does not allow for repeat analysis if needed.

Test Classification and CPT Code

Testosterone Totl: 84403

Testosterone Free: 84402

SHBG: 84270

Test classification: This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

LOINC

Testosterone Totl: 2986-8

Testosterone Free: 2991-8

Percent Free Testosterone: 15432-8

SHBG: 13967-5

Last Reviewed

08/2025