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Test Code LAB145 Amikacin Level, Random 

Performing Laboratory

St. Louis Children's Hospital - Chemistry

Specimen Requirements

Patient Preparation: None

Container/Tube  
Preferred:
Mint green-top Lithium Heparin (MNTGRN LiHep) tube
Acceptable: Lavender-top (EDTA) tube or plain red-top tube

Collection Volume: 2.0 mL (minimum 1.0 mL)

Submission Container/Tube: If specimen is coming from outside facility and collected in a microtainer, Lavender-top (EDTA) tube, or plain red-top tube aliquot processed sample into a plastic vial for transport (labeling with specimen type).
Special Instructions: Send to lab promptly.

Sample Rejection

Mislabeled or unlabeled specimens

Logistics

Test Availability: Monday-Sunday
Cut-off time: None; performed as received
Turnaround Time: Same day; 2 hours from receipt in performing lab

Reference Values

Therapeutic Range: No established reference interval

Critical Values: ≥75.0 mcg/mL

Critical test results will be automatically called back initially and every 24 hours thereafter.

Methodology

Immunoassay (Kinetic interaction of microparticles in solution (KIMS) as measured by changes in light transmission).

Limitations

NA

Additional Information

FOR SLCH CORE LABORATORY USE ONLY

Lab Processing instructions: Centrifuge specimen to separate. If specimen is collected in a plain, red-top or Lavender-top (EDTA) tube, aliquot sample into a Roche false-bottom tube for testing (labeling with specimen type).
Processed Volume: 0.3 mL plasma/serum
Analyte Stability:

Specimen Type Temperature Time
Whole Blood (unprocessed)  Ambient 8 hours
Plasma/Serum (processed) Refrigerated (preferred) 2 days
  Ambient  8 hours
  Frozen  4 weeks 

Test Classification and CPT Coding

80150

LOINC

35669-1

Last Reviewed

06/2025