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Test Code LAB124 Testosterone, Total, Serum

Additional Codes

Mayo Test ID
ZW59

Performing Laboratory

Esoterix Endocrinology thru Mayo Medical Laboratory

Specimen Requirements

Patient Preparation: None

Collection Container/Tube:

Preferred: Gold SST gel tube

Acceptable: Plain red-top tube

Submission Container/Tube: If specimen is coming from outside facility aliquot processed sample into a plastic vial for transport within 1 hour.

Specimen Collection Volume: 2.0 mL (minimum 1.0 mL)

Special Instructions: Send to lab promptly

NOTE: Minimum volume does not allow for repeat analysis if necessary

Sample Rejection

Mislabled or unlabeled specimens

Logistics

Test Availability: Monday-Sunday

Cut-off time: None

Days set-up: Monday-Saturday

Turnaround Time: 4-6 days

Reference Values

Reference Interval: 

PREMATURE INFANTS     MALE RANGE(ng/dL)      FEMALE RANGE(ng/dL)

26 – 28 Weeks, Day 4:            59 – 125                              5 – 16

31 – 35 Weeks, Day 4:            37 – 198                              5 – 22

FULL–TERM INFANTS

Newborns: 

Male: 75-400 ng/dL
    1 – 7 Months: Levels decrease rapidly the first week to 20 – 50 ng/dL, then increase to 60 – 400 ng/dL (Mean = 190) between 20 – 60 days. Levels then decline to prepubertal range of < 2.5 – 10 by seven months.

Female: 20-64 ng/dL
    1 – 7 Months: Levels decrease during the first month to <10 ng/dL and remain there until puberty.

PREPUBERTAL CHILDREN

Males (1 – 10 Years): < 2.5 – 10 ng/dL

Females (1 – 9 Years): < 2.5 – 10 ng/dL

PUBERTY
MALE       FEMALE      

TANNER STAGE

AGE (years)

RANGE (ng/dL)

 

TANNER STAGE

AGE (years)

RANGE (ng/dL)

 
1 <9.8 <2.5-10   1 <9.2 <2.5-10  
2 9.8-14.5 18-150   2 9.2-13.7 7-28  
3 10.7-15.4 100-320   3 10.0-14.4 15-35  
4 11.8-16.2 200-620   4 10.7-15.6 13-32  
5 12.8-17.3 350-970   5 118-18.6 20-38  

ADULTS ≥18 Years       RANGE (ng/dL)

Male:                                  264 – 916

Female:

  Premenopausal:                10 – 55

  Postmenopausal:               7 – 40

Critical Value: none established

 

Methodology

High Pressure Liquid Chromatography/Tandem Mass Spectrometry (HPLC/MS-MS)

Limitations

  • NA

Additional Information

FOR SLCH CORE LABORATORY USE ONLY

Lab Processing Instructions: Centrifuge specimen to separate within one hour of collection. Transfer specimen to a 5-mL screw-capped plastic transport vial.

Processed Volume: 0.8 mL (minimum 0.6 mL) serum

Transport Temperature: Frozen

Analyte Stability:

Specimen Type Temperature Time
Whole Blood (unprocessed)   Ambient 1 hour
Plasma/Serum (processed) Refrigerated  7 days
  Ambient  7 days
  Frozen (preferred)     5.5 years    

NOTES: 

1.

Specimen must be separated within 1 hour of collection and removed from gel tube into a plastic vial for transport.

2.

Test should not be performed at Mayo. Need to use wild code to send to Esoterix

Test Classification and CPT Code

84403

Test classification: This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

LOINC

2986-8

Last Reviewed

08/2025