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HelpTest Code LAB112 Alkaline Phosphatase Total, Blood
Performing Laboratory
St. Louis Children's Hospital Laboratory - Chemistry
Specimen Requirements
Note: This test is also available as part of LAB17 Comprehensive Metabolic Panel (CMP) and LAB20 Hepatic Function Panel. See Common Panel Test Combinations to avoid duplicate requests if needed.
Patient Preparation: None
Collection Container/Tube:
Preferred: Mint green-top Lithium Heparin (MNTGRN LiHep) tube
Acceptable: Green samplette/microtainer, plain red-top tube, or Gold SST gel tube
Submission Container/Tube: If specimen is coming from outside facility and collected in a microtainer or plain red-top tube aliquot processed sample into a plastic vial for transport (labeling with specimen type).
Specimen Collection Volume: 1.0 mL (minimum 0.6 mL)
Special Instructions: None
Sample Rejection
Mislabled or unlabeled specimens
Logistics
Test Availability: Monday-Sunday
Cut-off time: None, performed as received
Turnaround Time: Same Day; 2 hours from receipt in performing lab
Reference Values
Reference Interval:
| <3 years: | 110-320 Units/L |
| 3-9 years: | 140-420 Units/L |
| 9-15 years: | 130-550 Units/L |
| 15-20 years: | 70-260 Units/L |
| ≥ 20 years: | 40-130 Units/L |
Critical Value: None established
Methodology
Colorimetric assay in accordance with a standardized method
Limitations
- In very rare cases gammopathy, in particular type IgM (Waldenstrom's macroglobulinemia) may cause unreliable results.
Additional Information
FOR SLCH CORE LABORATORY USE ONLY
Lab Processing Instructions: Centrifuge to separate. If specimen is collected in microtainer; or plain red-top tube aliquot sample into a Roche false-bottom tube for testing (labeling aliquot with specimen type).
Processed Volume: 0.3 mL plasma/serum
Analyte Stability:
| Specimen Type | Temperature | Time |
|---|---|---|
| Whole Blood (unprocessed) | Ambient | 24 hours |
| Plasma/Serum (processed) | Refrigerated (preferred) | 4 days |
| Ambient | 4 days | |
| Frozen | 2 months |
Test Classification and CPT Code
84075
LOINC
6767-6
Last Reviewed
06/2025