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Test Code CCH2903500 Perforin/Granzyme B

Important Note

SPECIMEN SHOULD ONLY BE COLLECTED MONDAY-THURSDAY BETWEEN 1000-1600 ONLY. 

DO NOT COLLECT ANYTIME FRIDAY-SUNDAY OR THE DAY BEFORE/OF A HOLIDAY.

DO NOT COLLECT MONDAY-THURDAY BEFORE 1000 OR AFTER 1600

Additional Codes

EPIC: order Miscellaneous Lab Test (LAB000)

CCHMC test code: 2903500 (includes Intracellular expression of Perforin and Granzyme B in NK cells and CD8+CD57 cells.

Performing Laboratory

St. Louis Children's Hospital Sendout to Cincinnati Children's Hospital Medical Center

Specimen Requirements

Specimen Type: Blood

Patient Preparation: None

Container/Tube        

Preferred:  Lavender-top (EDTA) Vacutainer®

Acceptable: DARK green-top (Sodium Heparin) Vacutainer®
Collection Volume: 2.0 mL (minimum 1.0 mL)

Special Instructions: 

  • Immediately invert tube gently 8-10 times after collection to ensure proper mixing of blood and anticoagulant (this will avoid specimen clotting).
  • Keep specimen at room temperature and send to lab promptly.
  • Send a completed "CCHMC requisition" test requisition with specimen.

 

Sample Rejection

Specimen collected before 1000 or after 1600 Monday thru Thursday

Specimens collected anytime Friday-Sunday OR day of/before a Holiday

Specimens that are clotted

Specimens that are hemolyzed

Specimens collected in an unacceptable anticoagulant

Mislabeled or unlabeled specimens.

Logistics

Test Availability:  Monday-Thursday only with collection between 1000-1600.

Cut-off time: 1600

Days set up by performing lab: Monday-Friday

Turnaround Time: 3-5 days

Reference Values

Reference Interval: Interpretive report will be provided

Critical Value: None established

Methodology

Flow Cytometry

Limitations

This flow cytometry assay is intended to be used as a screening test. Screening tests are not 100% sensitive nor specific, and a normal result should not preclude molecular sequencing if a patient's clinical presentation suggests that the probability of a diagnosis is high

Additional Information

FOR SLCH CORE LABORATORY USE ONLY

Lab Processing Instructions: DO NOT SPIN. Keep specimen at room temperature

Processed Volume: 2.0 mL (minimum 1.0 mL) EDTA whole blood

Transport Temperature: Ambient
Analyte Stability: 

Specimen Type Temperature    Time   
Whole Blood (unprocessed)  Ambient 24 hours   
  Refrigerated    UNACCEPTABLE   
  Frozen UNACCEPTABLE

Note: 

  1. Specimens should be collected and shipped Mon-Thursday only by overnight express for delivery by 1000 next day. Must be received at CCHMC lab within 24 hours of collection AND if sent on Thursday MUST be in CBDI lab by 1100 am Friday.
  2. Send the completed "CCHMC DIL" test requisition with specimen.
  3. DO NOT transfer blood to other containers
  4. Specimen will be rejected if sample is frozen or refrigerated.

Test Classification and CPT Code

88184

88185x4

88187

LOINC

NA

Last Reviewed

10/2025