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FLUORESCENCE IN SITU HYBRIDIZATION (FISH)

Performing Laboratory

St. Louis Children's Hospital - Core Lab Sendouts

Specimen Requirements

Patient Preparation: None

Container/Tube  
Preferred:  GreenNaHep Vacutainer®.(DARK green-top no gel Sodium Heparin tube)
Collection Volume: 

AGE DEPENDENT

infants: 1-2 mL

children: 3-5 mL

adults: 5-10 mL

Special Instructions:

Immediately invert tube gently 8-10 times after collection to ensure proper mixing of blood and anticoagulant (this will avoid specimen clotting). Maintain at RT or refrigerate if sample cannot be delivered within 24 hours.

Send the EPIC Cytogenetics requisition that prints when order released with specimen.
NOTE: If specimen type is Bone Marrow to be sent to outside reference facility please order as a 'Miscellaneous Genetics Test" and see "Bone Marrow General Guidelines".

 

 

Sample Rejection

Clotted specimens

Specimens collected in other than Dark green-top NaHep (no gel) tubes.

Mislabeled or unlabeled specimens.

Logistics

Test Availability:

Daily

Turnaround Time:

varies by test
Aneuscreen: 3-5 days
Microdeletion testing: 5-7 days

Test set-up:

Monday-Friday

Lab Testing Section:

Send-outs

Referred to:

Cytogenetics and Molecular Pathology Laboratory at Washington University

Reference Values

Reference Interval: Interpretive report will be provided

Critical Value: None established

Limitations: NA

Referred to:  Cytogenetics and Molecular Pathology Laboratory at Washington University

Methodology

NA

Limitations

  • NA

Additional Information

FOR SLCH CORE LABORATORY USE ONLY

Lab Processing instructions:

DO NOT SPIN. Send in original vacutainer

 

Processed Volume:

AGE DEPENDENT

infants: 1-2 mL whole blood

children: 3-5 mL whole blood

adults: 5-10 mL whole blood

 

Transport Temperature:

Ambient

 

Analyte Stability:

NA
Maintain at ambient temperature or refrigerate if sample cannot be delivered within 24 hours. Samples should be no more than 48 hours old.

 

Notes:

Send the EPIC Cytogenetics order requisition with specimen
Call cytogenetics lab at 314-362-2258 or 314-454-8101 with any questions.
IF specimen is BM to go to outside reference facility make sure order is for Misc Genetics and follow that reference lab's processing/handling instructions

 

Test Classification and CPT Code

NA

LOINC

NA

Last Reviewed

07/2025